T-Coffeeisamultiplesequencealignmentserver.ItcanalignProtein,DNAandRNAsequences.YoucanuseT-Coffeetoalignsequencesortocombinetheoutputofyourfavoritealignmentmethodsintooneuniquealignment.Itisalsoabletocombinesequenceinformationwithproteinstructuralinformation,profileinformationorRNAsecondarystructures.

更新时间：2023-01-28 直链：tcoffee.vital-it.ch

FASTAfindsregionsoflocalsimilaritybetweensequences.Theprogramcomparesnucleotideorproteinsequencestosequencedatabasesandcalculatesthestatisticalsignificanceofmatches.

更新时间：2024-03-21 直链：fasta.bioch.virginia.edu

ClustalW2isageneralpurposemultiplesequencealignmentprogramforDNAorproteins.Itattemptstocalculatethebestmatchfortheselectedsequences,andlinesthemupsothattheidentities,similaritiesanddifferencescanbeseen.

更新时间：2023-01-28 直链：www.ebi.ac.uk

TheBasicLocalAlignmentSearchTool(BLAST)findsregionsoflocalsimilaritybetweensequences.Theprogramcomparesnucleotideorproteinsequencestosequencedatabasesandcalculatesthestatisticalsignificanceofmatches.BLASTcanbeusedtoinferfunctionalandevolutionaryrelationshipsbetweensequencesaswellashelpidentifymembersofgenefamilies.

更新时间：2024-03-21 直链：blast.ncbi.nlm.nih.gov